The condensed protocols from molecular cloning : a laboratory manual /
Sambrook, Joseph.
The condensed protocols from molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell. - Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2006. - v, 800 p. : ill. 28 cm.
Companion v. to: Molecular cloning / Joseph Sambrook, David W. Russell. 3rd ed. c2001.
Includes index.
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
9780879697716 (pbk. : alk. paper);0879697717 (pbk. : alk. paper)
Cloning, Molecular
QH 440.5 / .S26 2006
The condensed protocols from molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell. - Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2006. - v, 800 p. : ill. 28 cm.
Companion v. to: Molecular cloning / Joseph Sambrook, David W. Russell. 3rd ed. c2001.
Includes index.
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
9780879697716 (pbk. : alk. paper);0879697717 (pbk. : alk. paper)
Cloning, Molecular
QH 440.5 / .S26 2006