TY  - BOOK
AU  - Sambrook, Joseph.
AU  - Russell, David W.
TI  - The condensed protocols from molecular cloning: a laboratory manual 
SN  - 9780879697716 (pbk. : alk. paper);0879697717 (pbk. : alk. paper)
PY  - 2006///
CY  - Cold Spring Harbor, N.Y.
PB  - Cold Spring Harbor Laboratory Press
KW  - Cloning, Molecular
N1  - Companion v. to: Molecular cloning / Joseph Sambrook, David W. Russell. 3rd ed. c2001; Includes index; Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies
ER  -