000			03086nam a2200205Ia 4500
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020			_a9781588293794 (alk. paper);1588293793 (alk. paper);9781597450058 (e-ISBN);1597450057 (e-ISBN)
060			_aW1;QU 350 _bME9616J v.325 2006;.N83 2006
245		0	_aNuclear reprogramming : _bmethods and protocols / _cedited by Steve Pells.
260			_aTotowa, N.J. : _bHumana Press, _cc2006.
300			_axiv, 333 p. : _bill.
490			_aMethods in molecular biology,
504			_aIncludes bibliographical references and index.
505			_aNuclear transplantation in Xenopus / John B. Gurdon -- Nuclear transfer in sheep / William A. Ritchie -- Protocols for nuclear transfer in mice / Shaorong Gao -- Isolation of stromal stem cells from human adipose tissue / Andrew C. Boquest ... [et al.] -- Nuclear reprogramming by cell fusion / Robert H. Broyles ... [et al.] -- Polyethylene glycol-mediated cell fusion / Jian Yang and Ming Hong Shen -- Epigenetic reprogramming of somatic genomes by electrofusion with embryonic stem cells / Masako Tada and Takashi Tada -- Quantification of cell fusion by flow cytometry / Stephen Sullivan ... [et al.] -- Modulation of cell fate using nuclear and cytoplasmic extracts / Anne-Mari Ha??kelien, Kristine G. Gaustad, and Philippe Collas -- Transgenic systems in nuclear reprogramming / Megan Munsie, Peter Mountford, and Jennifer Nichols -- Using immunofluorescence to observe methylation changes in mammalian preimplantation embryos / Fa??tima Santos and Wendy Dean -- Observing S-phase dynamics of histone modifications with fluorescently labeled antibodies / Rong Wu, Anna V. Terry, and David M. Gilbert -- Quantitative analysis of telomerase activity and telomere length in domestic animal clones / Dean H. Betts ... [et al.] --;Pluripotency: capacity for in vitro differentiation of undifferentiated embryonic stem cells / Cornelia Wiese ... [et al.] -- Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens / Bruce A. Fenderson ... [et al.] -- Analysis of the nucleolar compartment of the nucleus as an indicator of nuclear reprogramming after nuclear transfer / Jacques-E. Fle??chon -- Methylation-sensitive polymerase chain reaction / Hannah R. Moore, Richard R. Meehan, and Lorraine E. Young -- Analysis of DNA methylation profiles in preimplantation embryos using bisulfite mutagenesis / Yong-Mahn Han, Seok-Ho Kim, and Yong-Kook Kang -- Chromatin immunoprecipitation assay for mammalian tissues / Fiona B. Turner, Wang L. Cheung, and Peter Cheung -- Histone modifications and transcription factor binding on chromatin: ChIP/PCR assays / Jaejoon Won and Tae Kook Kim -- In vivo genomic footprinting using LM-PCR methods / Hiromi Tagoh, Peter N. Cockerill, and Constanze Bonifer -- Analyzing histone modification using crosslinked chromatin treated with micrococcal nuclease / Pascal Lefevre and Constanze Bonifer.
650			_aCell Fusion
650			_aCell Nucleus
650			_aTransplantation
700			_aPells, Steve.
942			_bDIMC _cBK
999			_c29737 _d29737