000			01589nam a2200193Ia 4500
008			250806s9999||||xx |||||||||||||| ||und||
020			_a9780879697716 (pbk. : alk. paper);0879697717 (pbk. : alk. paper)
060			_aQH 440.5 _b.S26 2006
100			_aSambrook, Joseph.
245		4	_aThe condensed protocols from molecular cloning : _ba laboratory manual / _cJoseph Sambrook, David W. Russell.
260			_aCold Spring Harbor, N.Y. : _bCold Spring Harbor Laboratory Press, _cc2006.
300			_av, 800 p. : _bill. _c28 cm.
500			_aCompanion v. to: Molecular cloning / Joseph Sambrook, David W. Russell. 3rd ed. c2001.
504			_aIncludes index.
505			_aPlasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
650			_aCloning, Molecular
700			_aRussell, David W.
942			_bDIMC _cBK
999			_c31531 _d31531