images (140×140)   Dow University of Health Sciences Library Catalogue

Welcome to Dow University of Health Sciences Library Online Public Access Catalog

The condensed protocols from molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell.

By: Contributor(s): Material type: TextPublication details: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2006.Description: v, 800 p. : ill. 28 cmISBN:
  • 9780879697716 (pbk. : alk. paper);0879697717 (pbk. : alk. paper)
Subject(s): NLM classification:
  • QH 440.5
Contents:
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)

Companion v. to: Molecular cloning / Joseph Sambrook, David W. Russell. 3rd ed. c2001.

Includes index.

Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.

There are no comments on this title.

to post a comment.
Previous
Back to results
Next
Close



©2026, All rights reserved. Dow University of Health Sciences, Karachi, Pakistan.