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The condensed protocols from molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell.

By: Contributor(s): Material type: TextPublication details: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2006.Description: v, 800 p. : ill. 28 cmISBN:
  • 9780879697716 (pbk. : alk. paper);0879697717 (pbk. : alk. paper)
Subject(s): NLM classification:
  • QH 440.5
Contents:
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
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Holdings
Item type Current library Home library Call number Status Barcode
Books Dow International Medical College Dow International Medical College QH 440.5 .S26 2006 (Browse shelf(Opens below)) Available 00001864

Companion v. to: Molecular cloning / Joseph Sambrook, David W. Russell. 3rd ed. c2001.

Includes index.

Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 -- Working with high-capacity vectors -- Gas electrophoresisof DNA and pulsed-field agarose -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.

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